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71.
Frontispiece: New Dual Fluorescent Probe for Simultaneous Biothiol and Phosphate Bioimaging 下载免费PDF全文
72.
By integrating photoinduced electron transfer (PET) into the design of functional bioluminogenic probes, Urano and his coworkers recently developed a new rational design strategy, BioLeT. It is expected that this BioLeT strategy will enable us to design and develop new bioluminescence probes for detecting various biomolecules with no catalytic or reactive activity. 相似文献
73.
Dr. Damien Lhenry Dr. Manuel Larrouy Dr. Claire Bernhard Dr. Victor Goncalves Dr. Olivier Raguin Dr. Peggy Provent Dr. Mathieu Moreau Dr. Bertrand Collin Dr. Alexandra Oudot Dr. Jean‐Marc Vrigneaud Dr. François Brunotte Dr. Christine Goze Prof. Franck Denat 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(37):13091-13099
In molecular imaging, multimodal imaging agents can provide complementary information, for improving the accuracy of disease diagnosis or enhancing patient management. In particular, optical/nuclear imaging may find important preclinical and clinical applications. To simplify the preparation of dual‐labeled imaging agents, we prepared versatile monomolecular multimodal imaging probe (MOMIP) platforms containing both a fluorescent dye (BODIPY) and a metal chelator (polyazamacrocycle). One of the MOMIP was conjugated to a cyclopeptide (i.e., octreotide) and radiolabeled with 111In. In vitro and in vivo studies of the resulting bioconjugate were conducted, highlighting the potential of these BODIPY‐based bimodal probes. This work also confirmed that the biovector and/or the bimodal probes must be chosen carefully, due to the impact of the MOMIP on the overall properties of the resulting imaging agent. 相似文献
74.
Fluorescent Coumarin–Artemisinin Conjugates as Mitochondria‐Targeting Theranostic Probes for Enhanced Anticancer Activities 下载免费PDF全文
Dr. Xu Zhang Qian Ba Zhanni Gu Dr. Diliang Guo Prof. Yu Zhou Prof. Yungen Xu Prof. Hui Wang Prof. Deju Ye Prof. Hong Liu 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(48):17415-17421
Mitochondria‐targeting theranostic probes that enable the simultaneously reporting of and triggering of mitochondrial dysfunctions in cancer cells are highly attractive for cancer diagnosis and therapy. Three fluorescent mitochondria‐targeting theranostic probes have been developed by linking a mitochondrial dye, coumarin‐3‐carboximide, with a widely used traditional Chinese medicine, artemisinin, to kill cancer cells. Fluorescence images showed that the designed coumarin–artemisinin conjugates localized mainly in mitochondria, leading to enhanced anticancer activities over artemisinin. High cytotoxicity against cancer cells correlated with the strong ability to accumulate in mitochondria, which could efficiently increase the intracellular reactive oxygen species level and induce cell apoptosis. This study highlights the potential of using mitochondria‐targeting fluorophores to selectively trigger and directly visualize subcellular drug delivery in living cells. 相似文献
75.
Multifunctional Nano‐Bioprobes Based on Rattle‐Structured Upconverting Luminescent Nanoparticles 下载免费PDF全文
Dr. Shan Lu Dr. Datao Tu Ping Hu Jin Xu Renfu Li Dr. Meng Wang Dr. Zhuo Chen Prof. Mingdong Huang Prof. Dr. Xueyuan Chen 《Angewandte Chemie (International ed. in English)》2015,54(27):7915-7919
Lanthanide‐doped upconversion nanoparticles (UCNPs) have shown great promise in versatile bioapplications. For the first time, organosilica‐shelled β‐NaLuF4:Gd/Yb/Er nanoprobes with a rattle structure have been designed for dual‐modal imaging and photodynamic therapy (PDT). Benefiting from the unique rattle structure and aromatic framework, these nanoprobes are endowed with a high loading capacity and the disaggregation effect of photosensitizers. After loading of β‐carboxyphthalocyanine zinc or rose Bengal into the nanoprobes, we achieved higher energy transfer efficiency from UCNPs to photosensitizers as compared to those with conventional core–shell structure or with pure‐silica shell, which facilitates a large production of singlet oxygen and thus an enhanced PDT efficacy. We demonstrated the use of these nanoprobes in proof‐of‐concept X‐ray computed tomography (CT) and UC imaging, thus revealing the great potential of this multifunctional material as an excellent nanoplatform for cancer theranostics. 相似文献
76.
Synthesis and Evaluation of GdIII‐Based Magnetic Resonance Contrast Agents for Molecular Imaging of Prostate‐Specific Membrane Antigen 下载免费PDF全文
Dr. Sangeeta Ray Banerjee Dr. Ethel J. Ngen Matthew W. Rotz Dr. Samata Kakkad Ala Lisok Richard Pracitto Mrudula Pullambhatla Dr. Zhengping Chen Dr. Tariq Shah Dr. Dmitri Artemov Dr. Thomas J. Meade Dr. Zaver M. Bhujwalla Dr. Martin G. Pomper 《Angewandte Chemie (International ed. in English)》2015,54(37):10778-10782
Magnetic resonance (MR) imaging is advantageous because it concurrently provides anatomic, functional, and molecular information. MR molecular imaging can combine the high spatial resolution of this established clinical modality with molecular profiling in vivo. However, as a result of the intrinsically low sensitivity of MR imaging, high local concentrations of biological targets are required to generate discernable MR contrast. We hypothesize that the prostate‐specific membrane antigen (PSMA), an attractive target for imaging and therapy of prostate cancer, could serve as a suitable biomarker for MR‐based molecular imaging. We have synthesized three new high‐affinity, low‐molecular‐weight GdIII‐based PSMA‐targeted contrast agents containing one to three GdIII chelates per molecule. We evaluated the relaxometric properties of these agents in solution, in prostate cancer cells, and in an in vivo experimental model to demonstrate the feasibility of PSMA‐based MR molecular imaging. 相似文献
77.
Cover Picture: Velocity of a Molecule Evaporated from a Water Nanodroplet: Maxwell–Boltzmann Statistics versus Non‐Ergodic Events (Angew. Chem. Int. Ed. 49/2015) 下载免费PDF全文
Assist. Prof. Dr. Hassan Abdoul‐Carime Francis Berthias Dr. Linda Feketeová Dr. Mathieu Marciante Dr. Florent Calvo Dr. Valérian Forquet Prof. Dr. Henry Chermette Dr. Bernadette Farizon Prof. Dr. Michel Farizon Prof. Dr. Tilmann D. Märk 《Angewandte Chemie (International ed. in English)》2015,54(49):14587-14587
78.
Back Cover: Folding Up of Gold Nanoparticle Strings into Plasmonic Vesicles for Enhanced Photoacoustic Imaging (Angew. Chem. Int. Ed. 52/2015) 下载免费PDF全文
79.
80.
Rare Earth Ion Mediated Fluorescence Accumulation on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at the Single‐Cell Level 下载免费PDF全文
Xiaobo Zhang Prof. Dr. Chenghui Liu Honghong Wang Hui Wang Prof. Dr. Zhengping Li 《Angewandte Chemie (International ed. in English)》2015,54(50):15186-15190
A single microbead‐based fluorescence imaging (SBFI) strategy that enables detection of protein kinase activity from single cell lysates is reported. We systematically investigated the ability of various rare earth (RE) ions, immobilized on the microbead, for specific capturing of kinase‐induced phosphopeptides, and Dy3+ was found to be the most prominent one. Through the efficient concentration of kinase‐induced fluorescent phosphopeptides on a Dy3+‐functionalized single microbead, kinase activity can be detected and quantified by reading the fluorescence on the microbead with a confocal fluorescence microscope. Owing to the extremely specific recognition of Dy3+ towards phosphopeptides and the highly‐concentrated fluorescence accumulation on only one microbead, ultrahigh sensitivity has been achieved for the SBFI strategy which allows direct kinase analysis at the single‐cell level. 相似文献